cryptosporidium is an apicomplexan parasite of humans and a wild range of domestic as well as wild animals. an 833-bp fragment of the 18s-rrna gene was used to identify cryptosporidium spp. recovered from children and adult patients, in human immunodeficiency virus (hiv) positive and negative patients in iran. initial identification of cryptosporidiosis was carried out by ziehl-neelsen acid-fas...

background: mutations of gjb2 gene encoding connexion 26 are the most common cause of hearing loss in many popula­tions. a very wide spectrum of gjb2 gene mutations associated with hearing loss have been detected but pathogenic role has been tested only for a part of them. in this study, we have provided genetic evidence on the pathogenicity of our previ­ously reported novel gjb2 allelic varian...

Two Gram-negative, non-motile, non-spore-forming, rod-shaped strains, SW-2T and SW-26, were isolated from sea water of the East Sea in Korea. These organisms grew optimally at 37 degrees C and in the presence of 2-3 % (w/v) NaCl. They did not grow without NaCl or in the presence of >9 % (w/v) NaCl. Strains SW-2T and SW-26 were characterized chemotaxonomically as having MK-7 as the predominant i...

background: to differentiate sarcocystis macro-cyst-forming species in slaughtered sheep in babol area, mazandaran province, sequence analysis of 18s rrna gene was performed. methods: overall, 150 slaughtered sheep were examined macroscopically in slaughterhouse, babol and intra-abdominal and diaphragm muscles tissues infected with macro-cyst of sarcocystis spp. were collected in 2013. one macr...

In the present study basidiomycetous yeast species were characterised from soil samples in different regions of Iran. Soil samples were collected from 5–15 cm depth, subsequently yeasts were isolated and purified. Yeast isolates were identified using D1/D2 domains of 26S rRNA and physiologic tests. The results of this study revealed a rich diversity amongst basidiomycetous yeast species in the ...

rRNA genes are attractive targets for developing PCR assays targeting human fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed spacers, and the 5' end of the 28S rRNA gene. An approximately 2,900-bp region of the 28S rRNA gene remains largely unexplored because sequences of many medically relevant fungi are either unavailable or undefined in genomic databases...

The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacill...

The complete mitogenome of Fusarium oxysporum f. sp. albedinis (FOA), the causal agent destructive fusarium wilt in date palm, is sequenced and assembled. circular isolate Foa44 51,601 bp length contains 26 transfer RNA (tRNA) genes, one ribosomal (rRNA), 28 protein-coding genes. A mitogenome-based phylogenetic analysis revealed that FOA congruent with previous nuclear-gene results.

Background: Clarithromycin resistance in Helicbacter pylori has been found to be associated with point mutations in 23s rRNA gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. The aim of this study was to determine the most important point mutations in 23s rRNA gene in H. pylori that are closely related to clarith-romycin resistance amo...